TY  - JOUR
A1  - Vey, Chiara Theresa
A1  - Kaygusuz, Viola
A1  - Kayser, Josefa Sophia
A1  - Beyer, Andreas
A2  - WILEY, 
T1  - Detection and enzymatic characterization of human saliva amylase
T2  - Biochemistry and Molecular Biology Education
N2  - As a rule, an experiment carried out at school or in undergraduate study
courses is rather simple and not very informative. However, when the experiments
are to be performed using modern methods, they are often abstract and
difficult to understand. Here, we describe a quick and simple experiment,
namely the enzymatic characterization of ptyalin (human salivary amylase)
using a starch degradation assay. With the experimental setup presented here,
enzyme parameters, such as pH optimum, temperature optimum, chloride
dependence, and sensitivity to certain chemicals can be easily determined. This
experiment can serve as a good model for enzyme characterization in general,
as modern methods usually follow the same principle: determination of the
activity of the enzyme under different conditions. As different alleles occur in
humans, a random selection of test subjects will be quite different with regard
to ptyalin activities. Therefore, when the students measure their own ptyalin
activity, significant differences will emerge, and this will give them an idea of
the genetic diversity in human populations. The evaluation has shown that the
pupils have gained a solid understanding of the topic through this experiment.
KW  - Amylase, Enzymcharakterisierung
Y1  - 2024
UR  - https://whge.opus.hbz-nrw.de/frontdoor/index/index/docId/4494
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:1010-opus4-44942
VL  - 2024
PB  - Wiley
ER  -