TY  - JOUR
A1  - Ettelt, Volker
A1  - Ekkat, K.
A1  - Kämmerer, Peer W.
A1  - Kreikemeyer, Bernd
A1  - Epple, Matthias
A1  - Veith, Michael
T1  - Streptavidin-coated surfaces suppress bacterial colonisation by inhibiting non-specific protein adsorption
T2  - Journal of biomedical materials research : an official journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials. A
N2  - Streptavidin is a 58 kDa tetrameric protein with the highest known affinity to biotin with a wide range of applications in bionanotechnology and molecular biology. Dissolved streptavidin is stable at a broad range of temperature, pH, proteolytic enzymes and exhibits low non‐specific binding. In this study, a streptavidin monolayer was assembled directly on a biotinylated TiO2‐surface to investigate its stability against proteolytic digestion and its suppression of initial bacterial adsorption of Escherichia coli, Bacillus subtilis, and Streptococcus intermedius. In contrast to nonmodified TiO2 surfaces, streptavidin‐coated substrates showed only a negligible non‐specific protein adsorption at physiological protein concentrations as well as a significantly reduced bacterial adhesion. The antiadhesive properties were demonstrated to be the main reason for the suppression of bacterial adhesion, which makes this approach a promising option for future surface biofunctionalization applications.
Y1  - 2018
UR  - https://whge.opus.hbz-nrw.de/frontdoor/index/index/docId/2834
SN  - 1552-4965
VL  - 106
IS  - 3
SP  - 758
EP  - 768
PB  - New York
CY  - Wiley
ER  -