TY - JOUR A1 - Strotmann, Uwe A1 - Gendig, Cornelia A1 - Pastor Flores, Daniel A1 - Konrad, Odorico T1 - Bacterial Toxicity Testing: Modification and Evaluation of the Luminescent Bacteria Test and the Respiration Inhibition Test T2 - Processes N2 - The activated sludge respiration inhibition test and the luminescent bacteria test with Vibrio fischeri are important bacterial test systems for evaluation of the toxicity of chemical compounds. These test systems were further optimized to result in better handling, reliability and sensitivity. Concerning the Vibrio fischeri test, media components such as yeast extract and bivalent cation concentrations like Ca2+ and Mg2+ were optimized. The cultivation, storage conditions and reactivation process of the stored bacteria were also improved, which enabled simpler handling and led to good reproducibility. Additionally, the respiration inhibition test with a prolonged incubation time was further analyzed using different chlorinated phenols as reference compounds. It could be stated that a longer incubation period significantly improved the sensitivity of the test system. KW - bacterial toxicity KW - chlorinated phenols KW - Cr(VI) and Zn(II) cations KW - luminescent bacteria KW - respiration inhibition KW - activated sludge KW - long-term toxicity Y1 - 2020 UR - https://whge.opus.hbz-nrw.de/frontdoor/index/index/docId/3823 UR - https://nbn-resolving.org/urn:nbn:de:hbz:1010-opus4-38236 SN - 2227-9717 VL - 8 IS - 11 SP - 1349 ER -