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Streptavidin-coated TiO2 surfaces are biologically inert: Protein adsorption and osteoblast adhesion

  • Non‐fouling TiO2 surfaces are attractive for a wide range of applications such as biosensors and medical devices, where biologically inert surfaces are needed. Typically, this is achieved by controlled surface modifications which prevent protein adsorption. For example, polyethylene glycol (PEG) or PEG‐derived polymers have been widely applied to render TiO2 surfaces biologically inert. These surfaces have been further modified in order to achieve specific bio‐activation. Therefore, there have been efforts to specifically functionalize TiO2 surfaces with polymers with embedded biotin motives, which can be used to couple streptavidin for further functionalization. As an alternative, here a streptavidin layer was immobilized by self‐assembly directly on a biotinylated TiO2 surface, thus forming an anti‐adhesive matrix, which can be selectively bio‐activated. The anti‐adhesive properties of these substrates were analyzed by studying the interaction of the surface coating with fibronectin, lysozym, and osteoblast cells using surface plasmon resonance spectroscopy, atomic force microscopy, and light microscopy. In contrast to non‐modified TiO2 surfaces, streptavidin‐coated TiO2 surfaces led to a very biologically inert substrate, making this type of surface coating a promising alternative to polymer coatings of TiO2 surfaces.

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Metadaten
Author:Michael Lehnert, Miriam Gorbahn, Bilal Al-Nawas, Ingo Köper, Wolfgang Knoll, Michael Veith
DOI:https://doi.org/10.1002/jbm.a.33281
ISSN:1552-4965
Parent Title (English):Journal of biomedical materials research. an official journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials. A
Publisher:Wiley
Place of publication:New York
Document Type:Article
Language:English
Date of Publication (online):2018/07/12
Year of first Publication:2012
Publishing Institution:Westfälische Hochschule Gelsenkirchen Bocholt Recklinghausen
Release Date:2018/09/10
Volume:100A
Issue:2
First Page:388
Last Page:395
Departments / faculties:Institute / Westfälisches Institut für Gesundheit
Licence (German):License LogoEs gilt das Urheberrechtsgesetz

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