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Adsorption Conformation Behavior of Biotinylated Fibronectin on Streptavidin-Modified TiOx Surfaces Studied by SPR and AFM

  • It is well-known that protein-modified implant surfaces such as TiO2 show a higher bioconductivity. Fibronectin is a glycoprotein from the extracellular matrix (ECM) with a major role in cell adhesion. It can be applied on titanium oxide surfaces to accelerate implant integration. Not only the surface concentration but also the presentation of the protein plays an important role for the cellular response. We were able to show that TiOX surfaces modified with biotinylated fibronectin adsorbed on a streptavidin-silane self-assembly multilayer system are more effective regarding osteoblast adhesion than surfaces modified with nonspecifically bound fibronectin. The adsorption and conformation behavior of biotinylated and nonbiotinylated (native) fibronectin was studied by surface plasmon resonance (SPR) spectroscopy and atomic force microscopy (AFM). Imaging of the protein modification revealed that fibronectin adopts different conformations on nonmodified compared to streptavidin-modified TiOX surfaces. This conformational change of biotinylated fibronectin on the streptavidin monolayer delivers a fibronectin structure similar to the conformation inside the ECM and therefore explains the higher cell affinity for these surfaces.

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Metadaten
Verfasserangaben:Michael Lehnert, Miriam Gorbahn, Christopher Rosin, Marc Klein, Ingo Köper, Bilal Al-Nawas, Wolfgang Knoll, Michael Veith
DOI:https://doi.org/10.1021/la200908h
ISSN:1520-5827
ISSN:0743-7463
Titel des übergeordneten Werkes (Englisch):Langmuir
Verlag:ACS Publishing
Verlagsort:Washnigton DC
Dokumentart:Wissenschaftlicher Artikel
Sprache:Englisch
Datum der Veröffentlichung (online):10.09.2018
Datum der Erstveröffentlichung:20.03.2011
Veröffentlichende Institution:Westfälische Hochschule Gelsenkirchen Bocholt Recklinghausen
Datum der Freischaltung:10.09.2018
Jahrgang:27
Ausgabe / Heft:12
Erste Seite:7743
Letzte Seite:7751
Fachbereiche / Institute:Institute / Westfälisches Institut für Gesundheit
Lizenz (Deutsch):License LogoEs gilt das Urheberrechtsgesetz

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