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Layer-by-Layer Assembly of Streptavidin-Fibronectin-Multilayer on biotinylated TiOx

  • The biomodification of surfaces, especially titanium, is an important issue in current biomedical research. Regarding titanium, it is also important to ensure a specific protein modification of its surface because here protein binding that is too random can be observed. Specific nanoscale architectures can be applied to overcome this problem. As recently shown, streptavidin can be used as a coupling agent to immobilize biotinylated fibronectin (bFn) on a TiOX surface. Because of the conformation of adsorbed biotinylated fibronectin on a streptavidin monolayer, it is possible to adsorb more streptavidin and biotinylated fibronectin layers. On this basis, an alternating protein multilayer can be built up. In contrast to common layer-by-layer technology, in this procedure the mechanism of layer adsorption is very specific because of the interaction of biotin and streptavidin. In addition, we showed that the assembly of this multilayer system and its stability are dependent on the degree of labeling of biotinylated fibronectin. Hence we conclude that it is possible to build up well-defined nanoscale protein architectures by varying the degree of labeling of biotinylated fibronectin.

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Metadaten
Verfasserangaben:Michael Lehnert, Christopher Rosin, Wolfgang Knoll, Michael Veith
DOI:https://doi.org/10.1021/la303750p
Titel des übergeordneten Werkes (Englisch):Langmuir
Verlag:ACS Publishing
Verlagsort:Washnigton DC
Dokumentart:Wissenschaftlicher Artikel
Sprache:Englisch
Datum der Veröffentlichung (online):12.07.2018
Jahr der Erstveröffentlichung:2013
Veröffentlichende Institution:Westfälische Hochschule Gelsenkirchen Bocholt Recklinghausen
Datum der Freischaltung:04.09.2018
Jahrgang:29
Ausgabe / Heft:6
Erste Seite:1732
Letzte Seite:1737
Fachbereiche / Institute:Institute / Westfälisches Institut für Gesundheit
Lizenz (Deutsch):License LogoEs gilt das Urheberrechtsgesetz

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