Abstract It is thought that cells surviving ionizing radiation exposure repair DNA double-strand breaks (DSBs) and restore their genomes. However, the recent biochemical and genetic characterization of DSB repair pathways reveals that only homologous recombination (HR) can function in an error-free manner and that the non-homologous end joining (NHEJ) pathways canonical NHEJ (c-NHEJ), alternative end joining (alt-EJ), and single-strand annealing (SSA) are error-prone, and potentially leave behind genomic scars and altered genomes. The strong cell cycle restriction of HR to S/ G2 phases and the unparalleled efficiency of c-NHEJ throughout the cell cycle, raise the intriguing question as to how far a surviving cell reaches after repairing the genome back to its pre-irradiation state. Indeed, there is evidence that the genomes of cells surviving radiation treatment harbor extensive genomic alterations. To directly investigate this possibility, we adopted next-generation sequencing (NGS) technologies and tested a normal human fibroblast cell line, 82-6 hTert, after exposure up to 6 Gy. Cells were irradiated and surviving colonies expanded and the cells frozen. Sequencing analysis using the Illumina sequencing platform and comparison with the unirradiated genome detected frequent genomic alterations in the six investigated radiation survivor clones, including translocations and large deletions. Translocations detected by this analysis and predicted to generate visible cytogenetic alterations were frequently (three out of five) confirmed using mFISH cytogenetic analysis. PCR analysis of selected deletions also confirmed seven of the ten examined. We conclude that cells surviving radiation exposure tolerate and pass to their progeny a wide spectrum of genomic alterations. This recognition needs to be integrated into the interpretation of biological results at all endpoints, as well as in the formulation of mathematical models of radiation action. NGS analysis of irradiated genomes promises to enhance molecular cytogenetics by increasing the spectrum of detectable genomic alterations and advance our understanding of key molecular radiobiological effects and the logic underpinning DSB repair. However, further developments in the technology will be required to harness its full potential.

Abstract This case describes the difficulties of business succession from a successor’s perspective. The case is based on a real metalworking family business in an old industrial region in Germany. The key issues are challenges that, Michelle and Adrian, two second-generation successors within one company, face in teambuilding and finding their own place within the corporation and their team. The case is divided into two parts: Part 1 is a newspaper article about the succession process that points out stereotypes and writing styles in reporting on entrepreneurship/succession in the media. Part 2 is the succession story written from Michelle’s perspective and highlights challenges that female successors face in traditional male-dominated areas, for instance, when it comes to questions of balancing work and family needs. The case addresses issues relating to entrepreneurship and business succession classes, with sociological and psychological overtones.

Abstract Escape rooms have gained popularity across the globe, be it for entertainment, team building, or learning purposes. Solving puzzles has intrigued humanity since ancient times and playing games together facilitated intercultural encounters, encouraging mutual learning. This situated action builds on the idea of a mobile escape room within a suitcase, which when traveling shares previous encounters and collects new puzzles, thereby ever evolving. A suitcase will arrive in Sibu, Malaysia, with augmented reality stickers that entail puzzles created in Namibia. Visitors need to solve the puzzles to open the suitcase, which contains treats from Namibia, as well as materials to create new puzzles. Visitors participate in creating their own cultural puzzles, upload them to the augmented reality application, and thereby contribute to a new multicultural escape suitcase. The puzzles can also be played back at home, and the suitcase will continue evolving as it travels.

Abstract In this paper we present the co-design and implementation of an extended reality escape room with 26 primary school students. The aim of our study was to explore the co-design process with students and to co-create a playable escape room, providing an asymmetric immersive experience in which players collaborate. We realised the complexity of designing such an escape room with primary students. We share our experiences and learnings in regard to required capacities and skills of co-designers, and adjustment of complexity and timing to players. We also maintain that the integration of extended reality technologies into escape rooms requires further research to realise asymmetric co-located collaboration.

Mit der hier vorliegenden Toolbox stellen wir die Ergebnisse des dreijährigen, durch das Bundesministerium für Bildung und Forschung geförderten, Forschungsprojektes „Westfälische Erfinderinnen“ vor. Das Hauptziel des Projektes umfasste dabei, in den Regionen Ruhrgebiet und Münsterland Innovatorinnen zu identifizieren und die regionalen Mechanismen der (Un-)Sichtbarkeit von Innovatorinnen zu untersuchen und zu verstehen. Aufbauend auf den gewonnenen Erkenntnissen wurden ausgewählte Innovatorinnen zielgerichtet mit unterschiedlichen Kommunikationswerkzeugen ins Licht der regionalen Öffentlichkeit gerückt, um so das gesellschaftliche Bewusstsein für die Vielfalt von Innovationsprozessen und die Rolle von Innovatorinnen zu stärken. Die hier vorliegende Toolbox soll Hochschulen und andere zentrale Akteur:innen in weiteren Regionen ermutigen und anleiten, ihre Innovatorinnen zu finden, zu unterstützen und weithin sichtbar zu machen.